Recombinant Human CD50/ICAM-3 Protein (His & Fc Tag)
Recombinant Human CD50/ICAM-3 Protein (His & Fc Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically. ,125-135 kDa"]Product Description
CD50/ICAM-3 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD50/ICAM-3 ,Recombinant Proteins; predicted molecular weight: 77.2 kDa.
Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 77.2 kDa.
Database references: UniProt: NP_002153.2 | NCBI Gene: 3385 | Gene symbol: CD50/ICAM-3.
Research Use Only.
Key Facts
| Target | CD50/ICAM-3 |
| Also known as | CD50, CDW50, ICAM-3, ICAM-R |
| Species reactivity | Human |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Signal Transduction, immunology, |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.