Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)
Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 μg/mL. ,24 kDa"]Product Description
Sonic Hedgehog/SHH is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,Sonic Hedgehog/SHH ,Recombinant Proteins; predicted molecular weight: 21 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED<sub>50</sub> for this effect is typically 2-10 μg/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 21 kDa.
Database references: UniProt: Q15465 | NCBI Gene: 6469 | Gene symbol: Sonic Hedgehog/SHH.
Research Use Only.
Key Facts
| Target | Sonic Hedgehog/SHH |
| Also known as | HHG-1, SHH, Sonic Hedgehog Protein |
| Species reactivity | Human |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Cancer, Developmental Biology, epigenetics and nuclear signal, metabolism, Stem cells |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.