Recombinant Human IL4RA/CD124 Protein (His Tag)
Recombinant Human IL4RA/CD124 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human IL4R-His at 10 μg/mL (100 μl/well) can bind biotinylated human IL4. The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL. 2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The ED50 for this effect is 4-20 ng/ml in the presence of 2 ng/ml IL-4. ,43-48 kDa"]Product Description
IL4RA/CD124 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,IL4RA/CD124 ,Recombinant Proteins; predicted molecular weight: 25.3 kDa.
Quality and formulation: purity: > 98 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human IL4R-His at 10 μg/mL (100 μl/well) can bind biotinylated human IL4. The EC<sub>50</sub> of biotinylated human IL4 is 20.8-48.5 ng/mL. 2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The ED<sub>50</sub> for this effect is 4-20 ng/ml in the presence of 2 ng/ml IL-4.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 25.3 kDa.
Database references: UniProt: NP_000409.1 | NCBI Gene: 3566 | Gene symbol: IL4RA/CD124.
Research Use Only.
Key Facts
| Target | IL4RA/CD124 |
| Also known as | 582J, IL4R, CD124, IL-4RA, IL4RA, IL-4 receptor subunit alpha, IL-4-binding protein, IL4-BP, IL-4R subunit alpha, IL-4R-alpha, Interleukin-4 receptor subunit alpha, 582J2.1, IL-4R&, alpha, IL 4R alpha, IL4R nirs variant 1, IL4RA, Interleukin 4 receptor, Interleukin 4 receptor alpha chain, sIL4Ralpha/prot, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, Soluble interleukin-4 receptor subunit alpha |
| Species reactivity | Human |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Cancer, immunology |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.