Recombinant Human HtrA2/Omi Protein (His Tag)
Recombinant Human HtrA2/Omi Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in > 95% cleavage of β-casein, as revealed by SDS-PAGE. ,36.5 kDa"]Product Description
HtrA2/Omi is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: E.coli; physical form: Lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,HtrA2/Omi ,Recombinant Proteins; predicted molecular weight: 36.5 kDa.
Quality and formulation: purity: > 87 % as determined by reducing SDS-PAGE..
Functional evidence: activity: Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in > 95% cleavage of β-casein, as revealed by SDS-PAGE.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 36.5 kDa.
Database references: UniProt: O43464-1 | NCBI Gene: 27429 | Gene symbol: HtrA2/Omi.
Research Use Only.
Key Facts
| Target | HtrA2/Omi |
| Also known as | HTRA2, High temperature requirement protein A2, HtrA2, OMI, Omi stress-regulated endoprotease, PRSS25, Serine protease 25, Serine protease HTRA2, Serine proteinase OMI, mitochondrial |
| Species reactivity | Human |
| Purity | > 87 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Neuroscience, Cell Biology, Cancer, Metabolism |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.