Recombinant Human TNFR1/TNFRSF1A Protein (HEK293 Cells, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα. ,"]TNFR1/TNFRSF1A is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,TNFR1/TNFRSF1A ,Recombinant Proteins; predicted molecular weight: 22.7 kDa.
Quality and formulation: purity: > 92 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC<sub>50</sub> of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 22.7 kDa.
Database references: UniProt: NP_001056.1 | NCBI Gene: 7132 | Gene symbol: TNFR1/TNFRSF1A.
Research Use Only.
| Collaborator | Elabscience |
| Partner model | PKSH031378_100ug |
| Target | TNFR1/TNFRSF1A |
| Research areas | Signal Transduction, Cell biology, Cardiovascular, Cancer, immunology, Stem cells |
| Reactivity | Human |
| Purity | >92% by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Shelf life | 12 months |
| Availability | In stock - ships within 2 business days |
| Lead time | 2-3 business days |
| Category | Bioactivity | Binding | Cell-Based | In Vivo | ELISA Capture |
|---|---|---|---|---|---|
| Human |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
Environmental management certification.
Occupational health and safety management certification.
For research applications. Not for diagnostic use.
REACH compliant.
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