Recombinant Human RANKL/TNFSF11 Protein
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264. 7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.
Recombinant Human RANKL/TNFSF11 Protein supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human TNFSF11 at 2 μg/ml (100 μl/well) can bind biotinylated human Osteoprotegerin hFc, The EC50 of human Osteoprotegerin hFc is 5-40 ng/ml.2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264. 7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL. ,27 kDa"]
Product Description
RANKL/TNFSF11 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,RANKL/TNFSF11 ,Recombinant Proteins; predicted molecular weight: 20.5 kDa.
Quality and formulation: purity: > 85 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human TNFSF11 at 2 μg/ml (100 μl/well) can bind biotinylated human Osteoprotegerin hFc, The EC50 of human Osteoprotegerin hFc is 5-40 ng/ml.<br/>2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264. 7 cells. The ED<sub>50</sub> for this effect is typically 1.5-7.5 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 20.5 kDa.
Database references: UniProt: AAC51762.1 | NCBI Gene: 8600 | Gene symbol: RANKL/TNFSF11.
Research Use Only.
Key Facts
| Target | RANKL/TNFSF11 |
| Also known as | CD254, ODF, OPGL, OPTB2, Osteoclast differentiation factor, RANKL, Receptor activator of nuclear factor kappa-B ligand, TNFSF11, hRANKL2, sOdf, tumor necrosis factor ligand superfamily member 11 |
| Species reactivity | Human |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Immunology, Cardiovascular |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.