Recombinant Proteins RecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-15650

Recombinant Human ACE2 Protein (Fc Tag)

1. Using the Octet RED Systemthe affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 6 nM. 2. Using the Octet RED Systemthe affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 30 nM. 3. Using the Octet RED Systemthe affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) (ECDaa 1-1297) was 30 nM. 4. Using the Octet RED Systemthe affinity constant (Kd) of human AEC2-Fc bound to Spike-His (aa 1-760) was 10 nM.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human ACE2 Protein (Fc Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat: PKSR030500) on AMC Biosensor, can bind Recombinant Human ACE2 Protein , hFc Tag (Cat: PKSH030457) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) .
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Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat: PKSR030500) on AMC Biosensor, can bind Recombinant Human ACE2 Protein , hFc Tag (Cat: PKSH030457) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) .["1. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 6 nM. 2. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 30 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 30 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike-His (aa 1-760) was 10 nM. ,145-150 kDa"]

Product Description

ACE2 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,ACE2 ,Recombinant Proteins; predicted molecular weight: 110.3 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: 1. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 6 nM. 2. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 30 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 30 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike-His (aa 1-760) was 10 nM.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 110.3 kDa.

Database references: UniProt: NP_068576.1 | NCBI Gene: 59272 | Gene symbol: ACE2.

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: ACE2
Target ACE2
Also known as ACE-Related Carboxypeptidase, ACE2, ACEH, Angiotensin-Converting Enzyme 2, Angiotensin-Converting Enzyme Homolog, Metalloprotease MPROT15
Species reactivity Human
Purity > 95 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
Research area Cell biology, Cardiovascular
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
ACE2
Also known as
ACE-Related Carboxypeptidase, ACE2, ACEH, Angiotensin-Converting Enzyme 2, Angiotensin-Converting Enzyme Homolog, Metalloprotease MPROT15
Purity
> 95 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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