Recombinant Proteins RecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-15628

Recombinant Human GSK3B Protein (His Tag)

1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.Baculovirus-Insect Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human GSK3B Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (Cat: PKSH031171), EC<sub>50</sub> of biotinylated human HG3C-CTNNB1 (Cat: PKSH031171) is 0.15-0.35 μg/ml.
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Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (Cat: PKSH031171), EC<sub>50</sub> of biotinylated human HG3C-CTNNB1 (Cat: PKSH031171) is 0.15-0.35 μg/ml.["1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml. ,44-48 kDa"]

Product Description

GSK3B is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: Baculovirus-Insect Cells; physical form: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA; tag: Human ,GSK3B ,Recombinant Proteins; predicted molecular weight: 50.4 kDa.

Quality and formulation: purity: > 90 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.; concentration: Subject to label value..

Functional evidence: activity: 1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC<sub>50</sub> of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 50.4 kDa.

Database references: UniProt: NP_002084.2 | NCBI Gene: 2932 | Gene symbol: GSK3B.

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: GSK3B
Target GSK3B
Also known as GSK3B
Concentration Subject to label value.
Species reactivity Human
Purity > 90 % as determined by reducing SDS-PAGE.
Form / buffer Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA
Shelf life 6 months
Research area Signal Transduction, Cardiovascular, Neuroscience, Cancer, metabolism, Stem cells
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
GSK3B
Also known as
GSK3B
Concentration
Subject to label value.
Purity
> 90 % as determined by reducing SDS-PAGE.
Form / buffer
Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA

Storage & Stability

Shipped at
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Short-term storage
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Long-term storage
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
6 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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