Collaborator Elabscience
Recombinant ProteinsRecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-15628

Recombinant Human GSK3B Protein (His Tag)

1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.Baculovirus-Insect Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human GSK3B Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (Cat: PKSH031171), EC50 of biotinylated human HG3C-CTNNB1 (Cat: PKSH031171) is 0.15-0.35 μg/ml.
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Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (Cat: PKSH031171), EC50 of biotinylated human HG3C-CTNNB1 (Cat: PKSH031171) is 0.15-0.35 μg/ml.["1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml. ,44-48 kDa"]

Product Description

GSK3B is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: Baculovirus-Insect Cells; physical form: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA; tag: Human ,GSK3B ,Recombinant Proteins; predicted molecular weight: 50.4 kDa.

Quality and formulation: purity: > 90 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.; concentration: Subject to label value..

Functional evidence: activity: 1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC<sub>50</sub> of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 50.4 kDa.

Database references: UniProt: NP_002084.2 | NCBI Gene: 2932 | Gene symbol: GSK3B.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: GSK3B
Collaborator Elabscience
Partner model PKSH030425_50ug
Target GSK3B
Research areasSignal Transduction, Cardiovascular, Neuroscience, Cancer, metabolism, Stem cells
Reactivity Human
Concentration Subject to label value.
Purity >90% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 6 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Human

Specifications

Target
GSK3B
Also known as
GSK3B
Concentration
Subject to label value.
Purity
>90% by SDS-PAGE
Form / buffer
Liquid

Storage & Stability

Shipped at
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Short-term storage
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
6 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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