Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit

Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit - ABMIUM

Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit - ABMIUM
Manufactured by:Elabscience
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Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit

Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM11849-1
Product type
ELISA Kits
TargetNLRP3

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human NLRP3(NACHT, LRR and PYD domains-containing protein 3) ELISA Kit is a research-use ELISA kit from Elabscience for measuring NLRP3. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Immunology, Signal Transduction, Cancer, Cardiovascular, Neuroscience
Also known as
NLRP3,AGTAVPRL,AII,AVP,C1orf7,CIAS1,CLR1.1,FCAS,FCAS1,FCU,MWS,NALP3,PYPAF1
Cross-reactivity
This kit recognizes Human NLRP3 in samples.No significant cross-reactivity or interference between Human NLRP3 and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-NLRP3 capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NLRP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NLRP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NLRP3. You can calculate the concentration of Human NLRP3 in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-H2557_48T
Gene symbol
NLRP3
Gene ID
114548
UniProt accession
Q96P20
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

NLRP3NLR family pyrin domain containing 3

View target guide

NLRP3 identifies NLR family pyrin domain containing 3 in Homo sapiens (Human). This target record connects NCBI Gene 114548, UniProtKB Q96P20 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
114548
UniProtKB
Q96P20
Browse all NLRP3 productsRead target context & evidence
Elabscience
£363.00£254.10