25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience. Read more ›
["Standard curve"]25-hydroxyvitamin D3 (25-HVD3, calcidiol) is the principal circulating metabolite used to assess vitamin D status. It supports calcium and phosphate homeostasis by contributing to intestinal absorption, bone metabolism and renal mineral handling.
This kit uses a competitive ELISA format. The plate is pre-coated with 25-HVD3. Sample or standard analyte competes with the immobilised analyte for binding to a biotinylated 25-HVD3-specific antibody. After washing, avidin-HRP and TMB substrate generate a colour signal measured at 450 ± 2 nm. Signal intensity is inversely related to the 25-HVD3 concentration, which is calculated from the standard curve.
Sensitivity: 1.88 ng/mL. Detection range: 3.13–200 ng/mL. Elabscience lists serum, plasma and other biological fluids for this research-use-only assay.
Research Use Only. Not for diagnostic use.
| Collaborator | Elabscience |
| Partner model | E-EL-0015_48T |
| Target | 25-HVD3 |
| Research areas | Metabolism, Developmental biology |
| Cross-reactivity | This kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed |
| Assay type | Traditional ELISA Kits |
| Shelf life | 12 months from shipping dates |
| Availability | In stock - ships within 2 business days |
| Lead time | Ships same day if ordered before 12:00 UTC |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
Environmental management certification.
Occupational health and safety management certification.
For research applications. Not for diagnostic use.
REACH compliant.
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