NADP+/NADPH Colorimetric Assay Kit (WST-8)

NADP+/NADPH Colorimetric Assay Kit (WST-8) - ABMIUM

NADP+/NADPH Colorimetric Assay Kit (WST-8) - ABMIUM
Manufactured by:Elabscience
Save 20%ABMIUM Verified™RUO

NADP+/NADPH Colorimetric Assay Kit (WST-8)

NADP+/NADPH Colorimetric Assay Kit (WST-8) with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10640
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

NADP+/NADPH Colorimetric Assay Kit (WST-8) from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Oxidative Stress, Metabolic Diseases, Glycolysis and Lipid Metabolism, Cuproptosis, Disulfidptosis
Also known as
NADP,NADPH
Incubation temperature
This product can be stored at -20°C for 12 months with shading light.
Assay type
Assay Kits
Detection method
Colorimetric method
Technical note
Detect total content of NADP+ and NADPH:
Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm.
Detect NADPH:
After treating sample, heat at 60℃ water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm.
Detect NADP+ and NADP+/NADPH:
The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH.
Identifiers & database references
Supplier SKU / catalogue number
E-BC-K803-M_96T
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£317.00£253.60