Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-10196

Anti-Human CD47 Antibody [B6H12]

Anti-Human CD47 Antibody [B6H12] with collaborator-tested evidence for Flow Cyt. Read more ›

Jurkat were stained with 0.2 μg AF/LE Purified Anti-Human CD47 Antibody[B6H12] (Right) and 0.2 μg Mouse IgG21, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Mouse IgG Secondary Antibody.
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Jurkat were stained with 0.2 μg AF/LE Purified Anti-Human CD47 Antibody[B6H12] (Right) and 0.2 μg Mouse IgG21, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Mouse IgG Secondary Antibody.["FCM"]

Product Description

CD47 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Mouse; clonality: Monoclonal; clone: B6H12; isotype: Mouse IgG1, κ; available conjugation options: None (AF/LE), Unconjugated, FITC, PE, APC, PE/Cyanine 7, PerCP/Cyanine 5.5, Elab Fluor®647.

Application evidence: Elabscience lists this product for FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Human CD47 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: Q08722 | NCBI Gene: 961.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD47
Collaborator Elabscience
Partner model E-AB-F14130_100mg
Target CD47
Research areasImmunology, Cancer
Reactivity Human
Applications Flow Cytometry
ConjugationNone (AF/LE), Unconjugated, FITC, PE, APC, PE/Cyanine 7, PerCP/Cyanine 5.5, Elab Fluor®647
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Mouse
Clonality Monoclonal
Clone B6H12
Isotype Mouse IgG1, κ
Immunogen Recombinant Human CD47 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human

Specifications

Target
CD47
Also known as
MER, Protein MER, Leukocyte surface antigen CD, Antigenic surface determinant protein OA, CD47, IAP, MER6, OA3, Integrin-associated protein, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein (IAP), Protein MER6, Antigen identified by monoclonal 1D8, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, CD47 glycoprotein, CD47 molecule, Integrin Associated Protein, Integrin associated signal transducer, integrin-associated signal transducer), MER 6, OA 3, OTTHUMP00000041152, OTTHUMP00000041153, Rh related antigen, Surface antigen identified by monoclonal 1D8
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
B6H12
Isotype
Mouse IgG1, κ
Conjugation
None (AF/LE), Unconjugated, FITC, PE, APC, PE/Cyanine 7, PerCP/Cyanine 5.5, Elab Fluor®647
Species reactivity
Human
Tested applications
Flow Cytometry
Immunogen
Recombinant Human CD47 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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