Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-10175

Anti-Rat IL4 Antibody [5B3]

Anti-Rat IL4 Antibody [5B3] with collaborator-tested evidence for ICFCM. Read more ›

HEK293T cells transfected with pcDNA3.1 plasmid encoding Rat IL4 gene were stained with 0.2 μg Purified Anti-Rat IL4 Antibody[5B3] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Rat IgG Secondary Antibody.
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HEK293T cells transfected with pcDNA3.1 plasmid encoding Rat IL4 gene were stained with 0.2 μg Purified Anti-Rat IL4 Antibody[5B3] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Rat IgG Secondary Antibody.["ICFCM"]

Product Description

IL4 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Rat; host: Mouse; clonality: Monoclonal; clone: 5B3; isotype: Mouse IgG2a, κ; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for ICFCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Rat IL4 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P20096 | NCBI Gene: 287287.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: IL4
Collaborator Elabscience
Partner model E-AB-F13760P_100mg
Target IL4
Reactivity Rat
Applications ICFCM
ConjugationUnconjugated
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Mouse
Clonality Monoclonal
Clone 5B3
Isotype Mouse IgG2a, κ
Immunogen Recombinant Rat IL4 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIPICFCM
Rat

Specifications

Target
IL4
Also known as
Interleukin, B-cell growth factor, B-cell stimulatory factor, Lymphocyte stimulatory factor, BCGF1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BCGF-1, Binetrakin, BSF1, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra, Il4, BSF-1, Lymphocy, Il 4, B-cell growth factor 1, Il-4, IL4、BCGF-1、BCGF1、BSF-1、BSF1
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
5B3
Isotype
Mouse IgG2a, κ
Conjugation
Unconjugated
Species reactivity
Rat
Tested applications
ICFCM
Immunogen
Recombinant Rat IL4 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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