Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-10055

Anti-Human IFN-γ Antibody [B27]

Anti-Human IFN-γ Antibody [B27] with collaborator-tested evidence for ICFCM. Read more ›

HEK293T cells transfected with pcDNA3.1 plasmid encoding Human IFN-γ gene were stained with 0.2 μg AF/LE Purified Anti-Human IFN-γ Antibody[B27] (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by Elab Fluor®647-conjugated Goat Anti-Mouse IgG Secondary Antibody.
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HEK293T cells transfected with pcDNA3.1 plasmid encoding Human IFN-γ gene were stained with 0.2 μg AF/LE Purified Anti-Human IFN-γ Antibody[B27] (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by Elab Fluor®647-conjugated Goat Anti-Mouse IgG Secondary Antibody.["ICFCM"]

Product Description

IFN-γ is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Mouse; clonality: Monoclonal; clone: B27; isotype: Mouse IgG1, κ; available conjugation options: None (AF/LE), Unconjugated, FITC, PE, APC, Elab Fluor®700.

Application evidence: Elabscience lists this product for ICFCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Human IFN-γ protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P01579 | NCBI Gene: 3458.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: IFN-γ
Collaborator Elabscience
Partner model E-AB-F11960_100mg
Target IFN-γ
Research areasCell Biology, Immunology, Neuroinflammation, Neuroscience
Reactivity Human
Applications ICFCM
ConjugationNone (AF/LE), Unconjugated, FITC, PE, APC, Elab Fluor®700
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Mouse
Clonality Monoclonal
Clone B27
Isotype Mouse IgG1, κ
Immunogen Recombinant Human IFN-γ protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIPICFCM
Human

Specifications

Target
IFN-γ
Also known as
IFN-gamma, IFNG, IFNγ, Interferon gamma
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
B27
Isotype
Mouse IgG1, κ
Conjugation
None (AF/LE), Unconjugated, FITC, PE, APC, Elab Fluor®700
Species reactivity
Human
Tested applications
ICFCM
Immunogen
Recombinant Human IFN-γ protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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