Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-10030

Anti-Mouse CD14 Antibody [Sa14-2]

Anti-Mouse CD14 Antibody [Sa14-2] with collaborator-tested evidence for Flow Cyt. Read more ›

Mouse peritoneal macrophages were stained with 0.2 μg Purified Anti-Mouse CD279 Antibody[Sa14-2](Right) and 0.2 μg Rat IgG2a, κ Isotype Control(Left), followed by APC-conjugated Goat Anti-Rat IgG Secondary Antibody.
Zoom
Mouse peritoneal macrophages were stained with 0.2 μg Purified Anti-Mouse CD279 Antibody[Sa14-2](Right) and 0.2 μg Rat IgG2a, κ Isotype Control(Left), followed by APC-conjugated Goat Anti-Rat IgG Secondary Antibody.["FCM"]

Product Description

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion.

Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.

Product profile: reactivity: Mouse; host: Rat; clonality: Monoclonal; clone: Sa14-2; isotype: Rat IgG2a, κ; available conjugation options: Unconjugated, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®Violet 450, Elab Fluor®Red 780.

Application evidence: Elabscience lists this product for FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Mouse CD14 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P10810 | NCBI Gene: 12475.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD14
Collaborator Elabscience
Partner model E-AB-F1176A_100mg
Target CD14
Reactivity Mouse
Applications Flow Cytometry
ConjugationUnconjugated, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®Violet 450, Elab Fluor®Red 780
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Rat
Clonality Monoclonal
Clone Sa14-2
Isotype Rat IgG2a, κ
Immunogen Recombinant Mouse CD14 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Mouse

Specifications

Target
CD14
Also known as
CD 14, CD14 Antigen, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, Cd14, LPS R, Mo2
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Rat
Clonality
Monoclonal
Clone number
Sa14-2
Isotype
Rat IgG2a, κ
Conjugation
Unconjugated, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®Violet 450, Elab Fluor®Red 780
Species reactivity
Mouse
Tested applications
Flow Cytometry
Immunogen
Recombinant Mouse CD14 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

Customer Reviews

No customer reviews yet.