Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-10028

Anti-Human CD273 Antibody [24F.10C12]

Anti-Human CD273 Antibody [24F.10C12] with collaborator-tested evidence for Flow Cyt. Read more ›

HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD273 gene were stained with 0.2 μg AF/LE Purified Anti-Human CD273 Antibody[24F.10C12] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.
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HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD273 gene were stained with 0.2 μg AF/LE Purified Anti-Human CD273 Antibody[24F.10C12] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.["FCM"]

Product Description

CD273 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Mouse; clonality: Monoclonal; clone: 24F.10C12; isotype: Mouse IgG2a, κ; available conjugation options: None (AF/LE), Unconjugated.

Application evidence: Elabscience lists this product for FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Human CD273 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: Q9BQ51 | NCBI Gene: 80380.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD273
Collaborator Elabscience
Partner model E-AB-F11750_100mg
Target CD273
Research areasImmunology
Reactivity Human
Applications Flow Cytometry
ConjugationNone (AF/LE), Unconjugated
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Mouse
Clonality Monoclonal
Clone 24F.10C12
Isotype Mouse IgG2a, κ
Immunogen Recombinant Human CD273 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human

Specifications

Target
CD273
Also known as
PDL, PD-L, PD-1 ligand, PDCD1 ligand, Programmed death ligand, Programmed cell death 1 ligand, PDCD1L, bA574F, PDCD1LG, B7DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2, bA574F11.2, PDCD1LG2, B7-DC, Butyrophilin B7-DC, Programmed cell death 1 ligand 2, PD-1 ligand 2, PDCD1 ligand 2, Programmed death ligand 2, PDL2, PD-L2, B7 dendritic cell molecule, Butyrophilin B7 DC, Butyrophilin B7DC, CD 273, CD273 antigen, MGC142238, MGC142240, PD 1 ligand 2, PD L2, PD1 ligand 2, PD1L2, PDCD 1 ligand 2, PDL 2
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
24F.10C12
Isotype
Mouse IgG2a, κ
Conjugation
None (AF/LE), Unconjugated
Species reactivity
Human
Tested applications
Flow Cytometry
Immunogen
Recombinant Human CD273 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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