APC rabbit pAb from ELK Biotechnology
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APC rabbit pAb is a research-use antibody from ELK Biotechnology for studying APC in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008],
| Specificity | APC Polyclonal Antibody detects endogenous levels of APC protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,APC Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Galectin-3 Monoclonal Antibody(6G2)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western Blot analysis of various cells using APC Polyclonal Antibody diluted at 1:500 | Western Blot analysis of COLO205 cells using APC Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
APC (APC regulator of Wnt signaling pathway; adenomatous polyposis coli protein) is a multifunctional tumour-suppressor scaffold best known for restraining canonical Wnt signalling by promoting beta-catenin turnover.
Biological function: APC is a core component of the beta-catenin destruction machinery and promotes CTNNB1/beta-catenin degradation when canonical Wnt signalling is inactive. APC also associates with cytoskeletal systems, contributing to cell polarity, migration and cellular organisation.
