Manufactured by:ELK Biotechnology
RUO

Stat3 (phospho Tyr705) rabbit pAb

Stat3 (phospho Tyr705) rabbit pAb from ELK Biotechnology

SKU:
ABM104766
Product type
Primary Antibody
TargetStat3
ApplicationsWB, IHC, IF, ELISA, IP
Species / reactivityHuman, Mouse, Rat, Pig(Test by out customer)
Expression systemHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells (PubMed:31899195).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Stat3 (phospho Tyr705) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Stat3 in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat, Pig(Test by out customer). Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper

Additional antibody information

Specificity Phospho-Stat3 (Y705) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at Y705.
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>Chemokine signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Necroptosis, >>Signaling pathways regulating pluripotency of stem cells, >>JAK-STAT signaling pathway, >>Th17 cell differentiation, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>Insulin resistance, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Toxoplasmosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Viral carcinogenesis, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Pancreatic cancer, >>Acute myeloid leukemia, >>Non-small cell lung cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Inflammatory bowel disease, >>Lipid and atherosclerosis
Also known as
STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Tyr705. AA range:672-721
Observed band
88kD
Cellular localization
Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 672-721
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Pig(Test by out customer)
Identifiers & database references
Supplier SKU / catalogue number
ES1406
UniProt entry
P40763
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

STAT3signal transducer and activator of transcription 3

View target guide

STAT3 identifies signal transducer and activator of transcription 3 in Homo sapiens (Human). This target record connects NCBI Gene 6774, UniProtKB P40763 and related ABMIUM catalogue products.

Biological function: STAT3 is a signal transducer and transcription factor activated downstream of multiple cytokine and growth-factor receptors. Following phosphorylation and dimerisation, STAT3 can translocate to the nucleus and regulate gene expression controlling processes including proliferation, survival, differentiation and immune responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6774
UniProtKB
P40763
ELK Biotechnology
£220.00