Manufactured by:ELK Biotechnology
RUO

SCAMP1 rabbit pAb

SCAMP1 rabbit pAb from ELK Biotechnology

SKU:
ABM109576
Product type
Primary Antibody
TargetSCAMP1
ApplicationsWB, IHC
Species / reactivityHuman, Mouse, Rat
Expression systemWidely expressed, with highest expression in brain.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

SCAMP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SCAMP1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene product belongs to the SCAMP family of proteins, which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],

Additional antibody information

Specificity SCAMP1 Polyclonal Antibody detects endogenous levels of SCAMP1 protein.
Validation evidence captions Western blot analysis of lysates from NIH/3T3 cells, using SCAMP1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using SCAMP1 antibody. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
Antibody Research
Also known as
SCAMP1; SCAMP; Secretory carrier-associated membrane protein 1; Secretory carrier membrane protein 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Immunogen
The antiserum was produced against synthesized peptide derived from human SCAMP1. AA range:273-322
Observed band
32kD
Cellular localization
Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 273-322
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8050
UniProt entry
O15126
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00