mtTFA rabbit pAb from ELK Biotechnology
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mtTFA rabbit pAb is a research-use antibody from ELK Biotechnology for studying mtTFA in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
| Specificity | mtTFA Polyclonal Antibody detects endogenous levels of mtTFA protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,mtTFA Polyclonal Antibody(Green) was diluted at 1:200(4° overnight). Histone H3 (Tri Methyl Lys36) Monoclonal Antibody(Q12) (Red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500 | Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse | |||||||
| Bovine |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
