Manufactured by:ELK Biotechnology
RUO

CtBP1 rabbit pAb

CtBP1 rabbit pAb from ELK Biotechnology

SKU:
ABM108346
Product type
Primary Antibody
TargetCtBP1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in germinal center B-cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CtBP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CtBP1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CtBP1 Polyclonal Antibody detects endogenous levels of CtBP1 protein.
Validation evidence captions Western Blot analysis of Jurkat cells using CtBP1 Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CtBP1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat cells, using CtBP1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Wnt signaling pathway, >>Notch signaling pathway, >>Pathways in cancer, >>Chronic myeloid leukemia
Also known as
CTBP1; CTBP; C-terminal-binding protein 1; CtBP1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human CtBP1. AA range:388-437
Observed band
48kD
Cellular localization
Cytoplasm . Nucleus .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 388-437
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES4883
UniProt entry
Q13363
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00