Manufactured by:ELK Biotechnology
RUO

GK1 rabbit pAb

GK1 rabbit pAb from ELK Biotechnology

SKU:
ABM107090
Product type
Primary Antibody
TargetGK1
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GK1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GK1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011],

Additional antibody information

Specificity GK1 Polyclonal Antibody detects endogenous levels of GK1 protein.
Validation evidence captions Western Blot analysis of various cells using GK1 Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of HeLa cells, using GK Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 293 and Jurkat cells, using GK Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Glycerolipid metabolism, >>Metabolic pathways, >>PPAR signaling pathway
Also known as
GK; Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GK. AA range:461-510
Observed band
57kD
Cellular localization
Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 461-510
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2428
UniProt entry
P32189
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00