Manufactured by:ELK Biotechnology
RUO

Frizzled-9 rabbit pAb

Frizzled-9 rabbit pAb from ELK Biotechnology

SKU:
ABM107066
Product type
Primary Antibody
TargetFrizzled-9
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Monkey
Expression systemExpressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney. Expressed in neural progenitor cells (PubMed:27509850).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Frizzled-9 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Frizzled-9 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

frizzled class receptor 9(FZD9) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Frizzled-9 Polyclonal Antibody detects endogenous levels of Frizzled-9 protein.
Validation evidence captions Western Blot analysis of various cells using Frizzled-9 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using FZD9 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HUVEC, COLO, and COS cells, using FZD9 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using FZD9 antibody.
Key facts & specifications
Research areas
>>mTOR signaling pathway, >>Wnt signaling pathway, >>Hippo signaling pathway, >>Signaling pathways regulating pluripotency of stem cells, >>Melanogenesis, >>Cushing syndrome, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Basal cell carcinoma, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer
Also known as
FZD9; FZD3; Frizzled-9; Fz-9; hFz9; FzE6; CD antigen CD349
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FZD9. AA range:542-591
Observed band
64kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Relocalizes DVL1 to the cell membrane leading to phosphorylation of DVL1 and AXIN1 relocalization to the cell membrane. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 542-591
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES2374
UniProt entry
O00144
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00