Manufactured by:ELK Biotechnology
RUO

Frizzled-3 rabbit pAb

Frizzled-3 rabbit pAb from ELK Biotechnology

SKU:
ABM107065
Product type
Primary Antibody
TargetFrizzled-3
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemWidely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Frizzled-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Frizzled-3 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

frizzled class receptor 3(FZD3) Homo sapiens This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010],

Additional antibody information

Specificity Frizzled-3 Polyclonal Antibody detects endogenous levels of Frizzled-3 protein.
Validation evidence captions Western Blot analysis of various cells using Frizzled-3 Polyclonal Antibody | Western Blot analysis of RAW264.7 cells using Frizzled-3 Polyclonal Antibody | Immunofluorescence analysis of COS7 cells, using FZD3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A549 and RAW264.7 cells, using FZD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>mTOR signaling pathway, >>Wnt signaling pathway, >>Axon guidance, >>Hippo signaling pathway, >>Signaling pathways regulating pluripotency of stem cells, >>Melanogenesis, >>Cushing syndrome, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Basal cell carcinoma, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer
Also known as
FZD3; Frizzled-3; Fz-3; hFz3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FZD3. AA range:141-190
Observed band
76kD
Cellular localization
Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell surface . Apical cell membrane ; Multi-pass membrane protein. Colocalizes with FZD6 at the apical face of the cell (By similarity). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 141-190
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES2372
UniProt entry
Q9NPG1
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00