Manufactured by:ELK Biotechnology
RUO

NFAT1 (Phospho Ser326) rabbit pAb

NFAT1 (Phospho Ser326) rabbit pAb from ELK Biotechnology

SKU:
ABM106809
Product type
Primary Antibody
TargetNFAT1
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

NFAT1 (Phospho Ser326) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NFAT1 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012],

Additional antibody information

Specificity This antibody detects endogenous levels of Human,Mouse,Rat NFAT1 (Phospho Ser326)
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF-7,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
>>cGMP-PKG signaling pathway, >>Cellular senescence, >>Wnt signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Osteoclast differentiation, >>C-type lectin receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Oxytocin signaling pathway, >>Yersinia infection, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Human immunodeficiency virus 1 infection, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Lipid and atherosclerosis
Also known as
Nuclear factor of activated T-cells, cytoplasmic 2 (NF-ATc2;NFATc2;NFAT pre-existing subunit;NF-ATp;T-cell transcription factor NFAT1)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized peptide derived from human NFAT1 (Phospho Ser326)
Observed band
100kD
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES20145
UniProt entry
Q13469
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00