Manufactured by:ELK Biotechnology
RUO

CLC-4 rabbit pAb

CLC-4 rabbit pAb from ELK Biotechnology

SKU:
ABM106790
Product type
Primary Antibody
TargetCLC-4
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemAbundant in skeletal muscle and also detectable in brain and heart.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CLC-4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CLC-4 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

chloride voltage-gated channel 4(CLCN4) Homo sapiens The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012],

Additional antibody information

Specificity CLC-4 Polyclonal Antibody detects endogenous levels of CLC-4 protein.
Validation evidence captions Western Blot analysis of various cells using CLC-4 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of RAT-MUSLE cells using CLC-4 Polyclonal Antibody diluted at 1:500 | Immunofluorescence analysis of HUVEC cells, using CLCN4 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from MCF-7 cells, using CLCN4 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Neutrophil extracellular trap formation
Also known as
CLCN4; H(+)/Cl(-) exchange transporter 4; Chloride channel protein 4; ClC-4; Chloride transporter ClC-4
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human CLCN4. AA range:221-270
Observed band
85kD
Cellular localization
Early endosome membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 221-270
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1991
UniProt entry
P51793
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00