Manufactured by:ELK Biotechnology
RUO

ESX1 rabbit pAb

ESX1 rabbit pAb from ELK Biotechnology

SKU:
ABM105913
Product type
Primary Antibody
TargetESX1
ApplicationsWB
Species / reactivityHuman, Rat, Mouse
Expression systemExpressed in placenta and testis. Expressed in testicular germ cell tumors.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ESX1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ESX1 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a dual-function 65 kDa protein that undergoes proteolytic cleavage to produce a 45 kDa N-terminal fragment with a paired-like homeodomain and a 20 kDa C-terminal fragment with a proline-rich domain. The C-terminal fragment localizes to the cytoplasm while the N-terminal fragment localizes exclusively to the nucleus. In contrast to human, the mouse homolog has a novel PN/PF motif in the C-terminus and is paternally imprinted in placental tissue. This gene likely plays a role in placental development and spermatogenesis. [provided by RefSeq, Jan 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of ESX1 at Human
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Signaling pathways regulating pluripotency of stem cells
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human ESX1 AA range: 327-377
Cellular localization
Cytoplasm . Nucleus . ESXR1-N localizes specifically to the nucleus while ESXR1-C localizes specifically to the cytoplasm.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
45kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 327-377
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES16662
UniProt entry
Q8N693
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00