Manufactured by:ELK Biotechnology
RUO

PLC β3 (phospho Ser1105) rabbit pAb

PLC β3 (phospho Ser1105) rabbit pAb from ELK Biotechnology

SKU:
ABM105126
Product type
Primary Antibody
TargetPLCβ3
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemEpithelium,Uterus,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

PLC β3 (phospho Ser1105) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PLCβ3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

Additional antibody information

Specificity Phospho-PLC β3 (S1105) Polyclonal Antibody detects endogenous levels of PLC β3 protein only when phosphorylated at S1105.
Validation evidence captions Western Blot analysis of various cells using Phospho-PLC β3 (S1105) Polyclonal Antibody diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCB3 (Phospho-Ser1105) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PLCB3 (Phospho-Ser1105) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Inositol phosphate metabolism, >>Metabolic pathways, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>Chemokine signaling pathway, >>Phosphatidylinositol signaling system, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Wnt signaling pathway, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>NOD-like receptor signaling pathway, >>Circadian entrainment, >>Long-term potentiation, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Taste transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Melanogenesis, >>Thyroid hormone synthesis, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Glucagon signaling p
Also known as
PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PLCB3 around the phosphorylation site of Ser1105. AA range:1071-1120
Observed band
160kD
Cellular localization
Cytoplasm . Membrane . Nucleus . And particulate fractions. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 1071-1120
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1475
UniProt entry
Q01970
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00