Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

Primary Antibody supplied by Boster Bio

SKU:
ABM27091
Product type
Primary Antibodies
TargetRAC-alpha serine/threonine-protein kinase
ApplicationsWB
Species / reactivityChicken, Human, Mouse, Rat
PurityNo cross reactivity with other proteins.

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) is a research-use antibody from Boster Bio for studying RAC-alpha serine/threonine-protein kinase in WB workflows with listed reactivity for Chicken, Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Product information

Key facts & specifications
Also known as
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; ONCOGENE AKT1; PROTEIN KINASE B-ALPHA; RAC SERINE/THREONINE PROTEIN KINASE; Protein kinase B; Proto-oncogene c-Akt; RAC-PK-alpha
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: PKB-175
Isotype
Mouse IgG1
Cross-reactivity
No cross-reactivity with other proteins
Dilution range
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken
Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Observed band
60 kDa
Molecular weight
55735 MW
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification
Ascites
Form / buffer
Lyophilized
Sequence fragment
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Chicken
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
MA1085
Gene symbol
AKT1
Gene ID
Human(207), Mouse(11651), Rat(24185)
UniProt accession
P47196
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Storage warning
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00