Manufactured by:ELK Biotechnology
RUO

ACAT-1 rabbit pAb

ACAT-1 rabbit pAb from ELK Biotechnology

SKU:
ABM105537
Product type
Primary Antibody
TargetACAT-1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemAdipocyte,Brain,Fetal brain cortex,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ACAT-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACAT-1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009],

Additional antibody information

Specificity ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein.
Validation evidence captions Western Blot analysis of various cells using ACAT-1 Polyclonal Antibody | Western Blot analysis of A549 cells using ACAT-1 Polyclonal Antibody | Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody.
Key facts & specifications
Research areas
>>Fatty acid degradation, >>Valine, leucine and isoleucine degradation, >>Lysine degradation, >>Tryptophan metabolism, >>Pyruvate metabolism, >>Glyoxylate and dicarboxylate metabolism, >>Butanoate metabolism, >>Terpenoid backbone biosynthesis, >>Metabolic pathways, >>Carbon metabolism, >>Fatty acid metabolism, >>Fat digestion and absorption
Also known as
ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ACAT1. AA range:221-270
Observed band
45kD
Cellular localization
Mitochondrion .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 221-270
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1577
UniProt entry
P24752
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00