Manufactured by:ELK Biotechnology
RUO

Acetyl eIF5A/eIF5A2 (K47) rabbit pAb

Acetyl eIF5A/eIF5A2 (K47) rabbit pAb from ELK Biotechnology

SKU:
ABM103005
Product type
Primary Antibody
TargeteIF5A2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Acetyl eIF5A/eIF5A2 (K47) rabbit pAb is a research-use antibody from ELK Biotechnology for studying eIF5A2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,

Additional antibody information

Specificity Acetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47.
Validation evidence captions Western blot analysis of L929 lysis using antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from L929 cells, using eIF5A (Acetyl-Lys47) Antibody.
Key facts & specifications
Research areas
Antibody Research
Also known as
EIF5A2; Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60
Cellular localization
Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus, nuclear pore complex . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 11-60
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1096
UniProt entry
Q9GZV4
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00