Manufactured by:Elabscience
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Human mTOR(Mammalian Target of Rapamycin) ELISA Kit

Human mTOR(Mammalian Target of Rapamycin) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM11751-1
Product type
ELISA Kits
TargetmTOR

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human mTOR(Mammalian Target of Rapamycin) ELISA Kit is a research-use ELISA kit from Elabscience for measuring mTOR. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Also known as
FRAP,FRAP1,FRAP2,RAFT1,RAPT1,SKS,mTOR
Cross-reactivity
This kit recognizes Human mTOR in samples. No significant cross-reactivity or interference between Human mTOR and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-mTOR capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human mTOR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human mTOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human mTOR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human mTOR. You can calculate the concentration of Human mTOR in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-H1655_48T
Gene symbol
mTOR
Gene ID
2475
UniProt accession
P42345
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MTORmechanistic target of rapamycin kinase

View target guide

Mechanistic target of rapamycin kinase (MTOR), commonly called mTOR, is a serine/threonine protein kinase and core component of mTOR signalling complexes. This ABMIUM record connects NCBI Gene 2475 and UniProtKB P42345 to catalogue-backed research products.

Biological function: Mechanistic target of rapamycin kinase (mTOR/MTOR) is the catalytic kinase of mTORC1 and mTORC2. These complexes integrate nutrient, energy and growth-factor signals to regulate processes including protein synthesis, cell growth, metabolism, survival and cytoskeletal organisation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2475
UniProtKB
P42345
Elabscience
£363.00£254.10