Manufactured by:ELK Biotechnology
RUO

GADD34 rabbit pAb

GADD34 rabbit pAb from ELK Biotechnology

SKU:
ABM109742
Product type
Primary Antibody
TargetGADD34
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemBrain,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

GADD34 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GADD34 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity The antibody detects endogenous GADD34
Validation evidence captions Western blot analysis of MOUSE-BRAIN lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum
Also known as
Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Immunogen
Synthetic peptide from human protein at AA range: 430-490
Observed band
73kD
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side . Associates with membranes via an N-terminal amphipathic intramembrane region. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 430-490
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES8613
UniProt entry
O75807
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00