Manufactured by:ELK Biotechnology
RUO

Atg9a rabbit pAb

Atg9a rabbit pAb from ELK Biotechnology

SKU:
ABM109676
Product type
Primary Antibody
TargetAtg9a
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Embryo,Epithelium,Fetal brain,Lung endothelial cell,Lymph,Rectum tumor,Testis
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Atg9a rabbit pAb is a research-use antibody from ELK Biotechnology for studying Atg9a in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Plays a role in autophagy.,similarity:Belongs to the ATG9 family.,

Additional antibody information

Specificity Atg9a Polyclonal Antibody detects endogenous levels of Atg9a
Validation evidence captions Western Blot analysis of 293T 3T3 cells using Atg9a Polyclonal Antibody diluted at 1:800. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Autophagy - other, >>Mitophagy - animal, >>Autophagy - animal
Also known as
ATG9 autophagy related 9 homolog A (S. cerevisiae)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Immunogen
Synthetic Peptide of Atg9a
Observed band
85kD
Cellular localization
Preautophagosomal structure membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Mainly localizes to the trans-Golgi network (TGN) and the endosomal system; cycles between them though vesicle trafficking (PubMed:27316455, PubMed:27663665). Export from the TGN to promote formation of autophagosomes is mediated by the AP-4 complex (PubMed:29180427, PubMed:30262884). Under amino acid starvation or rapamycin treatment, redist
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8476
UniProt entry
Q7Z3C6
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ATG9Aautophagy related 9A

View target guide

ATG9A encodes a membrane phospholipid scramblase required for expansion of the forming autophagosome. This target record connects reviewed human ATG9A identifiers with ABMIUM catalogue products targeting ATG9A.

Biological function: ATG9A is a phospholipid scramblase central to autophagosome membrane expansion. It cycles between the phagophore assembly site and cytoplasmic vesicles, redistributing phospholipids across the bilayer so that membrane supplied during autophagosome growth can expand both leaflets. ATG9A also contributes to membrane trafficking events that support autophagosome initiation.

Organism
Homo sapiens (Human)
NCBI Gene
79065
UniProtKB
Q7Z3C6
ELK Biotechnology
£220.00