TMEM185A rabbit pAb from ELK Biotechnology
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TMEM185A rabbit pAb is a research-use antibody from ELK Biotechnology for studying TMEM185A in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is predicted to be a transmembrane protein. This gene is best known for localizing to the CpG island of the fragile site FRAXF. The 5' untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene, but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Aug 2013],
| Specificity | TMEM185A Polyclonal Antibody detects endogenous levels of TMEM185A protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of MCF7 cells, using TMEM185A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using TMEM185A Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using TMEM185A antibody. | Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200 |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
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Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
