Atg16 rabbit pAb
Manufactured by:ELK Biotechnology
RUO

Atg16 rabbit pAb

Atg16 rabbit pAb from ELK Biotechnology

SKU:
ABM109079
Product type
Primary Antibody
TargetAtg16
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemBrain,Colon,Epithelium,Fetal brain,Human lung,Mammary gland,Placenta,Small
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Atg16 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Atg16 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010],

Additional antibody information

Specificity Atg16 Polyclonal Antibody detects endogenous levels of Atg16 protein.
Validation evidence captions Western Blot analysis of Jurkat using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Autophagy - other, >>Autophagy - animal, >>NOD-like receptor signaling pathway, >>Shigellosis
Also known as
ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
Synthesized peptide derived from Atg16 . at AA range: 60-140
Observed band
70kD
Cellular localization
Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Lysosome membrane ; Peripheral membrane protein . Recruited to omegasomes membranes by WIPI2 (By similarity). Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures (By similarity). Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). Upon activation of non-canonical autophagy, recruited to single-membrane endolysosomal compartments (PubMed:29317426). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 60-140
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES6700
UniProt entry
Q676U5
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00