Manufactured by:ELK Biotechnology
RUO

SIRT2 rabbit pAb

SIRT2 rabbit pAb from ELK Biotechnology

SKU:
ABM108519
Product type
Primary Antibody
TargetSIRT2
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemIsoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. Isoform 2 is strongly expressed in the cortex, weakly expressed in heart and liver. Weakly expressed in several malignancies including breast, liver, brain, kidney and prostate cancers compared to normal tissues. Weakly expressed in glioma cell lines compared to normal brain tissues (at protein level). Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

SIRT2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SIRT2 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010],

Additional antibody information

Specificity SIRT2 Polyclonal Antibody detects endogenous levels of SIRT2 protein.
Validation evidence captions Western Blot analysis of 293 cells using SIRT2 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of SIRT2 Antibody. The lane on the right is blocked with the SIRT2 peptide. | Western blot analysis of the lysates from RAW264.7cells using SIRT2 antibody. | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>Nicotinate and nicotinamide metabolism, >>Metabolic pathways
Also known as
SIRT2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
The antiserum was produced against synthesized peptide derived from human SIRT2. AA range:321-370
Observed band
43kD
Cellular localization
Nucleus . Cytoplasm, perinuclear region . Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, spindle . Midbody . Chromosome . Perikaryon . Cell projection . Cell projection, growth cone . Myelin membrane . Localizes in the cytoplasm during most of the cell cycle except in the G2/M transition and during mitosis, where it is localized in association with chromatin and induces deacetylation of histone at 'Lys-16' (H4K16ac) (PubMed:17726514, PubMed:23468428). Colocalizes with KMT5A at mitotic foci (PubMed:23468428). Colocalizes with CDK1 at centrosome during prophase and splindle fibers during metaphase (PubMed:17488717). Colocalizes w
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 321-370
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5275
UniProt entry
Q8IXJ6
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SIRT2sirtuin 2

View target guide

SIRT2 identifies sirtuin 2 in Homo sapiens (Human). This target record connects NCBI Gene 22933, UniProtKB Q8IXJ6 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
22933
UniProtKB
Q8IXJ6
ELK Biotechnology
£220.00