Manufactured by:ELK Biotechnology
RUO

Ezh2 (phospho-Thr311) rabbit pAb

Ezh2 (phospho-Thr311) rabbit pAb from ELK Biotechnology

SKU:
ABM105901
Product type
Primary Antibody
TargetEzh2
ApplicationsWB
Species / reactivityHuman, Rat, Mouse
Expression systemIn the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level) (PubMed:31451685). Expressed in many tissues (PubMed:14532106). Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis (PubMed:14532106).
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Ezh2 (phospho-Thr311) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ezh2 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2) Homo sapiens This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Ezh2 (phospho-Thr311)
Key facts & specifications
Research areas
>>Lysine degradation, >>Metabolic pathways, >>MicroRNAs in cancer
Also known as
Histone-lysine N-methyltransferase EZH2 (EC 2.1.1.43) (ENX-1) (Enhancer of zeste homolog 2) (Lysine N-methyltransferase 6)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1000-2000
Immunogen
Synthesized phosho peptide around human Ezh2 (Thr311)
Observed band
82kD
Cellular localization
Nucleus . Localizes to the inactive X chromosome in trophoblast stem cells. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES16646
UniProt entry
Q15910
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EZH2enhancer of zeste 2 polycomb repressive complex 2 subunit

View target guide

EZH2 identifies enhancer of zeste 2 polycomb repressive complex 2 subunit in Homo sapiens (Human). This target record connects NCBI Gene 2146, UniProtKB Q15910 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
2146
UniProtKB
Q15910
ELK Biotechnology
£220.00