Cortactin (phospho Tyr466) rabbit pAb from ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
Cortactin (phospho Tyr466) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cortactin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
cortactin(CTTN) Homo sapiens This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010],
| Specificity | Phospho-Cortactin (Y466) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y466. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,Cortactin (phospho Tyr466) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. | Western Blot analysis of various cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of 293 cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
