Smad2 (phospho Ser467) rabbit pAb from ELK Biotechnology
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Smad2 (phospho Ser467) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Smad2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation
| Specificity | Phospho-Smad2 (S467) Polyclonal Antibody detects endogenous levels of Smad2 protein only when phosphorylated at S467. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Smad2 (S467) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of HepG2 cells using Phospho-Smad2 (S467) Polyclonal Antibody diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad2 (Phospho-Ser467) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad2 (Phospho-Ser467) Antibody. The picture on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
SMAD2 identifies SMAD family member 2 in Homo sapiens (Human). This target record connects NCBI Gene 4087, UniProtKB Q15796 and related ABMIUM catalogue products.
Biological function: SMAD family member 2 (SMAD2; MADH2) is a receptor-regulated SMAD transcriptional mediator in transforming growth factor beta (TGF-beta) and activin signalling. Activated type I receptors phosphorylate SMAD2, enabling complexes with SMAD4 that regulate target-gene transcription.
