Manufactured by:ELK Biotechnology
RUO

Nuclear Receptor NR4A1 (Phospho-Ser351) Rabbit pAb

Nuclear Receptor NR4A1 (Phospho-Ser351) Rabbit pAb from ELK Biotechnology

SKU:
ABM104976
Product type
Primary Antibody
TargetNur77
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemFetal muscle and adult liver, brain and thyroid.
PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Nuclear Receptor NR4A1 (Phospho-Ser351) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nur77 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],

Additional antibody information

Specificity This antibody detects endogenous pospho levels of human Nuclear Receptor NR4A1 (Phospho-Ser351)
Validation evidence captions Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.
Key facts & specifications
Research areas
>>MAPK signaling pathway, >>PI3K-Akt signaling pathway, >>Aldosterone synthesis and secretion, >>Cortisol synthesis and secretion, >>Cushing syndrome
Also known as
Nuclear receptor subfamily 4 group A member 1 (Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Immunogen
Synthesized pospho peptide derived from human Nuclear Receptor NR4A1 (Phospho-Ser351)
Observed band
77kD
Cellular localization
Nucleus . Cytoplasm . Mitochondrion . Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES14439
UniProt entry
P22736
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00