Manufactured by:ELK Biotechnology
RUO

POLK rabbit pAb

POLK rabbit pAb from ELK Biotechnology

SKU:
ABM104745
Product type
Primary Antibody
TargetPOLK
ApplicationsWB
Species / reactivityHuman, Mouse
Expression systemDetected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

POLK rabbit pAb is a research-use antibody from ELK Biotechnology for studying POLK in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of POLK at Human/Mouse
Validation evidence captions Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Fanconi anemia pathway, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Colorectal cancer, >>Pancreatic cancer, >>Endometrial cancer, >>Glioma, >>Thyroid cancer, >>Basal cell carcinoma, >>Melanoma, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human POLK AA range: 272-322
Cellular localization
Nucleus . Detected throughout the nucleus and at replication foci (PubMed:12414988). Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716). .
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
96kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 272-322
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES14031
UniProt entry
Q9UBT6
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00