Manufactured by:ELK Biotechnology
RUO

RAB7A rabbit pAb

RAB7A rabbit pAb from ELK Biotechnology

SKU:
ABM104636
Product type
Primary Antibody
TargetRAB7A
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemWidely expressed; high expression found in skeletal muscle.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

RAB7A rabbit pAb is a research-use antibody from ELK Biotechnology for studying RAB7A in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of RAB7A at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Mitophagy - animal, >>Autophagy - animal, >>Endocytosis, >>Phagosome, >>Salmonella infection, >>Amoebiasis, >>Tuberculosis
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human RAB7A AA range: 84-134
Cellular localization
Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Melanosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lipid droplet . Endosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle . Mitochondrion membrane ; Peripheral membrane protein . Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
23kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 84-134
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES13818
UniProt entry
P51149
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

RAB7ARAB7A, member RAS oncogene family

View target guide

RAB7A identifies RAB7A, member RAS oncogene family in Homo sapiens (Human). This target record connects NCBI Gene 7879, UniProtKB P51149 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
7879
UniProtKB
P51149
ELK Biotechnology
£220.00