GSK3β (phospho Ser9) rabbit pAb

Placeholder image

Placeholder image
Manufactured by:ELK Biotechnology
RUO

GSK3β (phospho Ser9) rabbit pAb

GSK3β (phospho Ser9) rabbit pAb from ELK Biotechnology

SKU:
ABM104440
Product type
Primary Antibody
TargetGSK3β
ApplicationsWB, IHC, IF, ELISA, IP
Species / reactivityHuman, Mouse, Rat, Drosophila
Expression systemExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GSK3β (phospho Ser9) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GSK3β in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat, Drosophila. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Additional antibody information

Specificity Phospho-GSK3β (S9) Polyclonal Antibody detects endogenous levels of GSK3β protein only when phosphorylated at S9.
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Chemokine signaling pathway, >>Cell cycle, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Wnt signaling pathway, >>Hedgehog signaling pathway, >>Axon guidance, >>Hippo signaling pathway, >>Focal adhesion, >>Signaling pathways regulating pluripotency of stem cells, >>IL-17 signaling pathway, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Neurotrophin signaling pathway, >>Dopaminergic synapse, >>Insulin signaling pathway, >>Melanogenesis, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>Cushing syndrome, >>Growth hormone synthesis, secretion and action, >>Alcoholic liver disease, >>Alzheimer disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Yersinia infection, >>Hepatitis C, >>Measles, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpes
Also known as
GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50
Observed band
48kD
Cellular localization
Cytoplasm . Nucleus . Cell membrane . The phosphorylated form shows localization to cytoplasm and cell membrane (PubMed:20937854). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 1-50
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Drosophila
Identifiers & database references
Supplier SKU / catalogue number
ES1322
UniProt entry
P49841
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00