Manufactured by:ELK Biotechnology
RUO

Calnexin (phospho Ser583) rabbit pAb

Calnexin (phospho Ser583) rabbit pAb from ELK Biotechnology

SKU:
ABM104160
Product type
Primary Antibody
TargetCalnexin
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemB-cell lymphoma,Epithelium,Fibroblast,Keratinocyte,Kidney,Liver,Lymph,Placenta,Platelet,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Calnexin (phospho Ser583) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Calnexin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583.
Validation evidence captions Western Blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1:2000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Calnexin (Phospho-Ser583) Antibody | Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum, >>Phagosome, >>Antigen processing and presentation, >>Thyroid hormone synthesis, >>Human T-cell leukemia virus 1 infection
Also known as
CANX; Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Calnexin around the phosphorylation site of Ser583. AA range:543-592
Observed band
90kD
Cellular localization
Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 543-592
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1276
UniProt entry
P27824
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00