Manufactured by:ELK Biotechnology
RUO

LDHA rabbit pAb

LDHA rabbit pAb from ELK Biotechnology

SKU:
ABM103680
Product type
Primary Antibody
TargetLDHA
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemPredominantly expressed in anaerobic tissues such as skeletal muscle and liver.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

LDHA rabbit pAb is a research-use antibody from ELK Biotechnology for studying LDHA in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008],

Additional antibody information

Specificity LDHA Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Glycolysis / Gluconeogenesis, >>Cysteine and methionine metabolism, >>Pyruvate metabolism, >>Propanoate metabolism, >>Metabolic pathways, >>HIF-1 signaling pathway, >>Glucagon signaling pathway, >>Central carbon metabolism in cancer
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from part region of human protein. AA range 51-71
Observed band
36kD
Cellular localization
Cytoplasm.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 51-71
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES11979
UniProt entry
P00338
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

LDHAlactate dehydrogenase A

View target guide

LDHA identifies lactate dehydrogenase A in Homo sapiens (Human). This target record connects NCBI Gene 3939, UniProtKB P00338 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3939
UniProtKB
P00338
ELK Biotechnology
£220.00