Manufactured by:ELK Biotechnology
RUO

BAG2 rabbit pAb

BAG2 rabbit pAb from ELK Biotechnology

SKU:
ABM102922
Product type
Primary Antibody
TargetBAG2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
Expression systemUterus,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

BAG2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying BAG2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity BAG2 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
23kD
Cellular localization
cytosol,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES10803
UniProt entry
O95816
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

BAG2BAG cochaperone 2

View target guide

BAG cochaperone 2 (BAG2), also called BAG family molecular chaperone regulator 2, is an HSP70/HSC70 co-chaperone that regulates the chaperone ATPase cycle. This target page groups ABMIUM catalogue reagents for studying BAG2-dependent chaperone control, proteostasis and cellular stress responses.

Biological function: BAG2 contains a BAG domain that binds HSP70-family chaperones and functions as a nucleotide-exchange factor, promoting ADP release during the HSP70/HSC70 chaperone cycle. Through this activity, BAG2 influences client-protein handling, folding and protein quality-control processes.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
9532
UniProtKB
O95816
ELK Biotechnology
£220.00