Manufactured by:ELK Biotechnology
RUO

Recombinant Mouse SHH (C25II)

Recombinant Mouse SHH (C25II) from ELK Biotechnology

SKU:
ABM100835
Product type
Recombinant Proteins
TargetSHH
ApplicationsProtein research
Purity>95% by SDS-PAGE

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Recombinant Mouse SHH (C25II) is a research-use recombinant protein from ELK Biotechnology preparation representing SHH supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198(Cys25Ile-Ile) is expressed.

Background

Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N­terminal fragment (Shh­N), and a catalytic­processing,glycosylated, 34 kDa, 239 aa C­terminal fragment. The 20 kDa Shh­N fragment is the core of the active hedgehog molecule. Mouse Shh­N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh­N, respectively.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Key facts & specifications
Research areas
Molecular Biology
Also known as
Sonic Hedgehog Protein; SHH; HHG-1; SHH
Assay type
Recombinant protein
Molecular weight
19.8 KDa
Form / buffer
Lyophilized
Endotoxin level
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Quantity per pack
50ug
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpecificationPurityEndotoxinBioactivity
Product QC
Identifiers & database references
Supplier SKU / catalogue number
EPT111
UniProt entry
Q62226
Storage, stability & shipping
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SHHsonic hedgehog signaling molecule

View target guide

SHH identifies sonic hedgehog signaling molecule in Homo sapiens (Human). This target record connects NCBI Gene 6469, UniProtKB Q15465 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6469
UniProtKB
Q15465
ELK Biotechnology
£523.00