Manufactured by:ELK Biotechnology
RUO

p53 Mouse mAb

p53 Mouse mAb from ELK Biotechnology

SKU:
ABM102275
Product type
Primary Antibody
Targetp53
ApplicationsWB, IHC, IF
Species / reactivityHuman

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

p53 Mouse mAb is a research-use antibody from ELK Biotechnology for studying p53 in WB, IHC, IF workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

Additional antibody information

Validation evidence captions Western blot analysis of 293T, with p53 mAb diluted at 1) 1:1,000 2) 1:2,000 | IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb(6C4)diluted at 1:200. | IF analysis of Hela with EM1052(Left) and DAPI (Right) diluted at 1:100.
Key facts & specifications
Research areas
Antibody Research
Also known as
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Dilution range
WB 1:2,000 IHC 1:200 IF 1:100-200
Immunogen
Synthetic Peptide
Observed band
53kDa
Cellular localization
Cytoplasm; Cytoplasm. Nucleus
Assay type
Antibody validation/application
Source organism
Mouse
Concentration
1mg/mL
Working concentration
WB 1:2,000 IHC 1:200 IF 1:100-200
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Identifiers & database references
Supplier SKU / catalogue number
EM1052
UniProt entry
P04637
Storage, stability & shipping
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage warning
Avoid repeated freeze-thaw cycles.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00