Manufactured by:ELK Biotechnology
RUO

GAPDH Mouse mAb

GAPDH Mouse mAb from ELK Biotechnology

SKU:
ABM102252
Product type
Primary Antibody
TargetGAPDH
ApplicationsWB, IHC, IF
Species / reactivityHuman, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GAPDH Mouse mAb is a research-use antibody from ELK Biotechnology for studying GAPDH in WB, IHC, IF workflows with listed reactivity for Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Additional antibody information

Validation evidence captions Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000. | Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), with GAPDH mouse mAb(2B8) diluted at 1:10,000.
Key facts & specifications
Research areas
Antibody Research
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Dilution range
WB 1:5,000 IHC 1:200
Immunogen
Synthetic Peptide
Observed band
37kDa
Cellular localization
Cytoplasm,Cytoskeleton,Membrane,Nucleus
Assay type
Antibody validation/application
Source organism
Mouse
Concentration
1mg/mL
Working concentration
WB 1:5,000 IHC 1:200
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Monkey
Dog
Chicken
Hamster
Rabbit
Pig
Sheep
Insect
Yeast
Identifiers & database references
Supplier SKU / catalogue number
EM1028
UniProt entry
P04406
Storage, stability & shipping
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage warning
Avoid repeated freeze-thaw cycles.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GAPDHglyceraldehyde-3-phosphate dehydrogenase

View target guide

GAPDH identifies glyceraldehyde-3-phosphate dehydrogenase in Homo sapiens (Human). This target record connects NCBI Gene 2597, UniProtKB P04406 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2597
UniProtKB
P04406
ELK Biotechnology
£220.00